What do #Electrophysiologists who record raw data from many channels (#NeuroPixels, #Hyperdrives…) do for data backup and storage? I just bought 2 x 4Tb external drives and it looks like they won’t be enough (at all). Is there a cheap and reliable AND huge storage way to do this?
What is the smallest optimal distance between independent #Tetrodes in dorsal CA1 (so that you don’t record from the same neuron in adjacent tetrodes and also movement of one doesn’t influence the movement of others)?
Say, using 12.7 μm diameter wire?
If MINI2P is good and open-source and reasonable cost-wise.. what reason would anyone have for pursuing 1P GRIN lens miniscope imaging any further? Any thoughts?
I haven't dug much further yet but my main hesitance would be just that I haven't seen it set up and used in labs outside of the developers' yet. Often there are implicit tricks and expertise that turn out to be important when transferring a technique.
My dream goal on here is to attract enough #Electrophysiologists so that we can debate things like which drives to use, nichrome or platinum wire, what gold-plating impedance, Vaseline or kwik-sil for surgery, tetrodes in or out of brain, going backwards during screening or not, which is the best recording system, rats vs mice (rats, ofc), etc.
So please tell your #Ephys colleagues to join! (Or they can also join the #NeuroMethods slack but that’s less fun)
If you have an academic email you could try to create an account with that and it might work, because it has some pre-approved domains.
If that doesn’t work, I’ll be happy to send you an invite link by direct message.
The #NeuroMethods slack is a platform where scientists can ask technical questions to other scientists. In general there will be at least 1 person who can answer your question - although we do need more #Ephys#Electrophysiology people on there!
thinking about making a set of dlx creOFF AAV constructs a la @JDimidschstein and @sohallab. before i do - has anyone got a stash? initially gfp / chr2 but also likely switch gfp/dtomato too. for some 'NOT x' experiments. would be nice if they already existed!
thinking about making a set of dlx creOFF AAV constructs a la @JDimidschstein and @sohallab. before i do - has anyone got a stash? initially gfp / chr2 but also likely switch gfp/dtomato too. for some 'NOT x' experiments. would be nice if they already existed! #neuroscience#neuromethods#aav#mouse#interneurons HT @elduvelle for the hashtags!