Sorry to learn of the passing of Prof. Alan Waggoner here in #Pittsburgh a couple of days ago.
He was an all round terrific person, besides being a great scientist. He developed the #Fluorescent Cyanine dyes (Cy3 Cy5 and others in the series) that had a significant impact on biological #imaging , in the 1990s
We report a fluorinated azido-coumarin scaffold which enables #photolabeling under fast and mild activation, and which can leave a #fluorescent tag on crosslinked species. Coupling this scaffold to an α-fucoside, we demonstrate fluorogenic photolabeling of #glycan-#protein interactions over a wide range of affinities. We expect this strategy to be broadly applicable to other #chromophores and we envision that such “fluoro-crosslinkers” could become important tools