#AdaptiveOptics (AO) was developed to wipe out atmospheric aberrations from astronomical observations. The same technique is now being used in #microscopy to illuminate biological specimens.
Microscopic dendrites of sodium hydrogencarbonate a.k.a. baking soda, viewed under a do-it-yourself smartphone-based microscope 🔬 in reflected white light.
Many thanks to my co-authors Leanna, Satya, Jesse, Leong, and to the entire @AICjanelia team for their feedback!
Every stage of a #microscopy project requires decisions, and those decisions are prone to #bias. In this paper, we use illustrative examples to describe potential pitfalls, and also provide strategies for mitigating bias.
Are you doing cell biology or biomedical research? Light microscopy-based imaging? If a facility for (multiplexed) imaging would pop up in your department, what would be your expectations and wishes for it? If you (would) run one, what is crucial?
I’ve applied for a (multiplexed) imaging facility manager job and would like to collect a few use scenarios (think tools, training, data analysis) to prepare for the interview. #CellBiology#Microscopy#Imaging#Biology#LightMicroscopy#Science
#Janelia is offering a 12-day bootcamp designed to demonstrate how biological queries and hypotheses steer experimental designs on various #microscopy platforms and across length scales from molecules to small animals:
@NikaShilobod@paleofire@geoscience With counting you mean like volume percentage in a soil sample? Back in the day we had a mechanical point counter in the lab for that. Today you'd rather train a neural net to do an image analysis I guess? Something like that?
Or is it more about chemical component determination?
@NikaShilobod@paleofire@geoscience I see that great info was already provided! In case this is done with age-dated material and accumulation rates should later be calculated (and other common evaluations of the charcoal data), there are several nice scripts (e.g. "CharAnalysis" for Matlab by Higuera et al., https://www.publish.csiro.au/wf/wf09134). Inspired by that, we made an R script with similar functions - the linked paper also includes more info on charcoal prep in the lab: https://zenodo.org/records/4943274
Hey @paleofire, I have a student that I am helping with his dissertation project and he needs to count #charcoal. Do you guys have any go to texts for lab and counting methods? I have not done this type of analysis before. #wildfire#palaeoecology#paleoecology#microscopy
This is a render of a #microscopy image.
The rendering has been done on #blender using the tif to blender plugin developped by Oane Gros.
The tutorial is very well done and make it easy for beginners to get incredible results very quickly.
Oh, wow, what a warm reception! the fediverse science community may be small but is definitely welcoming!
As many suggested, here is my #introduction : I am a physicist developing optical microscopy methods for biomedical applications at the Politecnico di Milano University, in Italy. I will try to fill the fediverse with colorful images of squishy biological things. Apparently, I am also the new resident #opendata manager, so expect some posts on that! #microscopy#lightsheet#optics
